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Structured Review

Promega rq1 dnase
Rq1 Dnase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rq1+dnase/rq1+dnase/pmc10536974__Data_Sheet_1-16-7-9
Average 90 stars, based on 1 article reviews
rq1 dnase - by Bioz Stars, 2026-09
90/100 stars

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Mutagenesis:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. To assay each non-luminescent polypeptide mutant induced culture, 30 μl of assay lysis buffer (25 mM HEPES pH 7.4, 0.3× Passive Lysis Buffer (Promega Corporation)), 0.006 U/μl RQ1 DNase (Promega Corporation) and 1× Peptide Solution (the relative concentration of the peptides were determined as explained in Example 2; from the relative concentration determined, the peptides were diluted to 1× in the lysis buffer) containing either the peptide fragment GVTGWRLCKRISA (SEQ ID NO: 18) or GVTGWRLFKRISA (SEQ ID NO: 106) were aliquoted into wells of a 96-well assay plate (Costar 3355). ..

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. To assay each mutant culture, 30 μl of assay lysis buffer (50 mM HEPES pH 7.5, 0.3× Passive Lysis Buffer (Promega Corporation)) and 0.006 U/μl RQ1 DNase (Promega Corporation)) containing non-luminescent peptide (1:10 dilution of NLpep9-HT (NLpep9 is SEQ ID NO: 17 and 18; HT is HaloTag E. coli clarified lysate) was added. .. The samples were shaken at room temperature for 10 minutes, and then 50 μl NANOGLO Luciferase Assay Reagent (Promega Corporation) was added.

Lysis:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. To assay each non-luminescent polypeptide mutant induced culture, 30 μl of assay lysis buffer (25 mM HEPES pH 7.4, 0.3× Passive Lysis Buffer (Promega Corporation)), 0.006 U/μl RQ1 DNase (Promega Corporation) and 1× Peptide Solution (the relative concentration of the peptides were determined as explained in Example 2; from the relative concentration determined, the peptides were diluted to 1× in the lysis buffer) containing either the peptide fragment GVTGWRLCKRISA (SEQ ID NO: 18) or GVTGWRLFKRISA (SEQ ID NO: 106) were aliquoted into wells of a 96-well assay plate (Costar 3355). ..

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. To assay each mutant culture, 30 μl of assay lysis buffer (50 mM HEPES pH 7.5, 0.3× Passive Lysis Buffer (Promega Corporation)) and 0.006 U/μl RQ1 DNase (Promega Corporation)) containing non-luminescent peptide (1:10 dilution of NLpep9-HT (NLpep9 is SEQ ID NO: 17 and 18; HT is HaloTag E. coli clarified lysate) was added. .. The samples were shaken at room temperature for 10 minutes, and then 50 μl NANOGLO Luciferase Assay Reagent (Promega Corporation) was added.

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. The pellet generated was then resuspended in 80 ml Peptide Lysis Buffer (25 mM HEPES pH 7.4, 0.1× Passive Lysis Buffer (Promega Corporation), 1 ml/ml lysozyme and 0.03 U/μl RQ1 DNase (Promega Corporation)) and incubated at room temperature for 15 minutes. ..

Concentration Assay:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. To assay each non-luminescent polypeptide mutant induced culture, 30 μl of assay lysis buffer (25 mM HEPES pH 7.4, 0.3× Passive Lysis Buffer (Promega Corporation)), 0.006 U/μl RQ1 DNase (Promega Corporation) and 1× Peptide Solution (the relative concentration of the peptides were determined as explained in Example 2; from the relative concentration determined, the peptides were diluted to 1× in the lysis buffer) containing either the peptide fragment GVTGWRLCKRISA (SEQ ID NO: 18) or GVTGWRLFKRISA (SEQ ID NO: 106) were aliquoted into wells of a 96-well assay plate (Costar 3355). ..

Electroporation:

Article Title: Splice-site variant c.3531+1G>T in COL1A1 in a family with osteogenesis imperfecta
Article Snippet: .. After electroporation, the cells were harvested after immediately culturing at 37°C with 5% CO 2 in pre-warmed DMEM supplemented with 10% fetal bovine serum for 48 h. Cells were processed for total RNA isolation using TRIzol and treated with RQ1 DNase (cat. no. M6101; Promega Corporation) to remove DNA according to the manufacturer's protocols. cDNA was synthesized using a RT kit (cat. no. R323-01; Vazyme Biotech Co., Ltd.), according to the manufacturer's protocol. ..

Isolation:

Article Title: Splice-site variant c.3531+1G>T in COL1A1 in a family with osteogenesis imperfecta
Article Snippet: .. After electroporation, the cells were harvested after immediately culturing at 37°C with 5% CO 2 in pre-warmed DMEM supplemented with 10% fetal bovine serum for 48 h. Cells were processed for total RNA isolation using TRIzol and treated with RQ1 DNase (cat. no. M6101; Promega Corporation) to remove DNA according to the manufacturer's protocols. cDNA was synthesized using a RT kit (cat. no. R323-01; Vazyme Biotech Co., Ltd.), according to the manufacturer's protocol. ..

Synthesized:

Article Title: Splice-site variant c.3531+1G>T in COL1A1 in a family with osteogenesis imperfecta
Article Snippet: .. After electroporation, the cells were harvested after immediately culturing at 37°C with 5% CO 2 in pre-warmed DMEM supplemented with 10% fetal bovine serum for 48 h. Cells were processed for total RNA isolation using TRIzol and treated with RQ1 DNase (cat. no. M6101; Promega Corporation) to remove DNA according to the manufacturer's protocols. cDNA was synthesized using a RT kit (cat. no. R323-01; Vazyme Biotech Co., Ltd.), according to the manufacturer's protocol. ..

other:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: Eluted protein was dialyzed into 25 mM Tris pH 7.5 and 25 mM NaCl and purified over HiTrap Q FF column (GE Healthcare) with 25 mM Tris pH 7.5 and 25 mM NaCl loading buffer and eluted with 25 mM Tris pH 7.5 and 125 mM NaCl.

Generated:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. The pellet generated was then resuspended in 80 ml Peptide Lysis Buffer (25 mM HEPES pH 7.4, 0.1× Passive Lysis Buffer (Promega Corporation), 1 ml/ml lysozyme and 0.03 U/μl RQ1 DNase (Promega Corporation)) and incubated at room temperature for 15 minutes. ..

Incubation:

Article Title: Activation of bioluminescence by structural complementation
Article Snippet: .. The pellet generated was then resuspended in 80 ml Peptide Lysis Buffer (25 mM HEPES pH 7.4, 0.1× Passive Lysis Buffer (Promega Corporation), 1 ml/ml lysozyme and 0.03 U/μl RQ1 DNase (Promega Corporation)) and incubated at room temperature for 15 minutes. ..

Article Title: GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins
Article Snippet: Visualization was done using an Amersham Imager 680 (GE Life Sciences Chicago, Illinois, USA). .. Following incubation, 2 μL of 10✕ RQ1 DNase Buffer, 6 μL of 50% PEG4000, and 1U RQ1 DNase (Promega, Madison, Wisconsin, USA) was added to the expression reaction, and nuclease-free water was added to make a final volume of 20 μL. ..

Expressing:

Article Title: GRASP: a modular toolkit for building synthetic pentatricopeptide repeat RNA-binding proteins
Article Snippet: Visualization was done using an Amersham Imager 680 (GE Life Sciences Chicago, Illinois, USA). .. Following incubation, 2 μL of 10✕ RQ1 DNase Buffer, 6 μL of 50% PEG4000, and 1U RQ1 DNase (Promega, Madison, Wisconsin, USA) was added to the expression reaction, and nuclease-free water was added to make a final volume of 20 μL. ..

Sonication:

Article Title: The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint.
Article Snippet: After washing the cells in ice-cold 1X PBS and collecting by centrifugation at 750g for 3 min at 4 ◦ , cells were lysed on ice for 5 min in RSB100T (100 mM NaCl, 10 mM Tris [pH 7.5], 2.5 mM MgCl 2 , 0.5% Triton X-100) supplemented with 1 mM CaCl 2 , 1 mM PMSF, and 1X Protease Inhibitor Cocktail Set V (Millipore 539137). .. The samples were sonicated once for 10 s at 20% amplitude on a Sonics Vibra cell model VCX130PB with model CV188 probe and then 10 U of RQ1 DNase were added (Promega M610A) for 15 min at room temperature while nutating. ..



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